Coding

Part:BBa_K1518000:Design

Designed by: Ian Valentine McDermott   Group: iGEM14_UCC_Ireland   (2014-09-28)


Thread Biopolymer Filament Gamma TBFG_EPTST


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 895


Design Notes

Sequence is codon optimised for expression in E. coli.


Source

The part DNA was artificially synthesised based on the mRNA sequence from Koch, et al (1994).

Protein is originally expressed by Pacific Hagfish (Eptatretus stoutii). DNA sequence was codon optimised using codon optimisation software and synthesised by Gen9.

References